Life goes deeper
The living
landscape all around us is just a thin veneer atop the vast, little-understood
bulk of the Earth’s interior. A widespread misconception about the deep
subsurface is that this realm consists of a continuous mass of uniform
compressed solid rock. Few are aware that this mass of rock is heavily
fractured, and water runs in many of these fractures and faults, down to depths
of many kilometres. The deep Earth supports an entire biosphere, largely cut
off from the surface world, and is still only beginning to be explored and
understood.
The amount of
water in the subsurface is considerable. Globally, the freshwater reservoir in
the subsurface is estimated to be up to 100 times as great as all the available
fresh water in the rivers, lakes and swamps combined. This water, ranging in
ages from seven years to 2 billion years, is being intensely studied by
researchers because it defines the location and scope of deep life. We know now
that the deep terrestrial subsurface is home to one quintillion simple (prokaryotic)
cells. That is two to 20 times as many cells as live in all the open ocean. By
some estimates, the deep biosphere could contain up to one third of Earth’s
entire biomass.
To comprehend
the deep biosphere, we must look past the familiar rules of biology. On the
surface, life without the Sun for an extended period of time is dangerous or
deadly. Without daylight, no plants or crops can grow. Temperatures get colder
and colder. Few organisms, including human beings, can long tolerate such
conditions. For instance, people living within the Arctic Circle – as well as
the maintenance staff at Antarctic research stations during winter – experience
24-hour darkness for several months each year. They are more vulnerable to
health issues such as depression. They find ways to adapt and get through the
long, dark, cold winter, but it isn’t easy.
Now imagine the
challenges in places that have been isolated from sunlight and organic
compounds derived from light-dependent reactions for millions or even billions
of years. It seems incomprehensible that anything could survive there. Yet
scientists, including the members of our team at Princeton University in New
Jersey, have found surprisingly diverse microorganisms in the deep Earth,
adapted to a lifestyle independent of the Sun.
Sunlight can
filter down to depths of about 1,000 metres in ocean water, but light
penetrates no more than a few centimetres into soils or rocks. Cold is not a
problem down there, however. Quite the opposite: rainwater that percolates
kilometres deep into the crust along fractures and faults between rocks can
reach temperatures of 60°C (140°F) or higher. The further down you go from the
surface, the closer you are to the mantle. Heat rising from the inner Earth is
what warms the fissure water. Additionally, the water is under high pressure,
contains very little or no oxygen, and is bombarded by radiation from natural
radioactive elements in the rocks.
Within this
hellish environment, though, are crucial ingredients for nurturing life.
Underground water reacts with minerals in the continental crust, and the longer
the water has been trapped down there, the more time there has been for the
results of those reactions to accumulate along the flow path. The slow
reactions between water and rock dissolve minerals into the water, and break up
some of the water molecules, producing molecular hydrogen. This hydrogen is an
important fuel for microorganisms in the deep subsurface.
We are also
beginning to map the different ecosystems and populations of the deep Earth.
Generally speaking, the older subterranean fissure water is brinier (saltier)
and has higher concentrations of dissolved hydrogen. Our studies and those by
some of our colleagues have shown an apparent trend that the microbes living in
older, more brackish water are distinctly different from ones in the younger,
less saline water.
Old-water
ecosystems are dominated by hydrogen-utilising microorganisms such as
sulphate-reducing bacteria and methane-producing archaea. Those
methane-producing archaea, or methanogens, are microbes that visually resemble
bacteria but are so structurally and genetically distinct that they belong to a
completely separate domain of life. Sulfate-reducing bacteria and methanogens
are among the life forms that appeared earlier in the evolutionary history. In
contrast, young-water ecosystems are dominated by metabolically diverse and
versatile bacteria of the phylum proteobacteria.
Studies of the
deep ecosystem are already resonating across many fields of science. They are sparking
new ideas about the origin of life and about the limits of metabolism. They are
filling in new details about the cycling, distribution and storage of carbon on
Earth. Deep continental ecosystems will aid the search for underground life on
rocky planets such as Mars; deep-sea and sub-seafloor ecosystems, in turn, will
help researchers assess the likelihood and possible nature of organisms living
on the ocean moons Europa and Enceladus. The implications of this research are
truly cosmic in scope.
Final del
formulario
Subsurface
microorganisms are estimated to be extraordinarily long-lived. In our studies,
they show a turnover time as slow as 1,000 years, meaning that they divide only
once every few thousand years. To put it in perspective, the common gut
bacterium E.coli divides once every 20 minutes. One of the
long-standing questions is, how do the deep microbes achieve such a slow-motion
lifestyle?
It is not easy
to make a living in the subsurface because the biochemical reactions to harness
energy from minerals and geological gases – a set of processes known as
chemotrophy – are not as efficient as photosynthesis, the process that green
plants use to capture energy from photons of sunlight on the surface. Some subsurface
microorganisms can form stress-resistant spores and remain inactive in order to
withstand extreme subsurface conditions; otherwise, microorganisms have to
invest at least a certain amount of energy, which varies from one taxa
(evolutionary population) to another, to maintain the integrity and
functionality of the cells.
Nowadays,
genetic sequencing techniques allow us to investigate in great detail which
organism has the potential to metabolise what component of the environment. We
can also probe the metabolic potential of the community as a whole using
metagenomics, a way to study the collective genetic diversity. Together, these
approaches are revealing the overall structure and functioning of the deep
biome.
Our studies of
the proteobacteria-dominated communities (collected from several sites 1 to 3
km below land surface) show that they share a high degree of similarity with
each other, as determined by a genetic marker known as the 16S ribosomal RNA.
However, the same functional traits are carried out by different taxa. This
variation cannot be explained by physical separation of the sites, nor by each
location’s unique physico-chemical features – normally the most ecologically
influential factors for such segregation. Neither depth nor water-residence
time appear to be a significant contributor to differences, either. Future
investigations on the origins of subsurface microorganisms, along with their
evolution and movement over the geological history, will aid our understanding
of the biogeography, or living landscape, of the subsurface.
Deep microbial
groups have established strong, paired metabolic partnerships
We recently
completed a study of subsurface microbes using high-throughput sequencing to
look at the total population of RNA and proteins. In a 2015 paper, we
described for the first time the comprehensive network of metabolic functions
being actively executed in the subsurface. At 1.3 km below land surface at the
Beatrix gold mine in South Africa, the active community was comprised of 39
phyla from all three domains of life: bacteria, archaea and eukarya – the
domain of complex organisms that include humans. Overall, the ecosystem was
dominated by proteobacteria.
The molecular
data, together with isotope geochemistry and thermodynamic modelling, presented
a unified story that the most successful group down there is the
betaproteobacteria, a class of proteobacteria that obtain energy through a
coupling of nitrate reduction and sulphur oxidation in order to fix carbon
dioxide for cellular growth. The demand for nitrate among deep microbes was
unexpected; it had gone unnoticed prior to our study because the measured
nitrate concentrations in the subsurface water samples were tiny. More
interesting, we deduce that deep microbial groups have established strong,
paired metabolic partnerships, or syntrophic relationships, which helps the
organisms overcome the challenges of extracting the limited energy that
originated from rocks. Rather than competing directly with each other, these
microbes establish a win-win collaboration.
Most of the
carbon in microbial cells appears to be derived directly and indirectly from
methane. This is true even though methanogens and methane-oxidising
microorganisms together accounted for less than 1 per cent of the organisms in our
samples – an astonishingly low fraction, given that methane was the most
abundant dissolved gas (~80 per cent) in the water samples we studied. The
different kinds of microbial taxa that recycle methane in the subsurface occur
at varying abundance over time and space.
Despite the
advantages of metabolic partnerships, some deep microbes have evolved to go it
alone. Through metagenomics and genome-based analysis, the research scientist
Dylan Chivian of Lawrence Berkeley National Laboratory (building on work by
Tullis Onstott, the head of our team at Princeton University) discovered a
sulphate-reducing bacterium, Candidatus Desulforudis audaxviator, that has
complete self-reliance for living in the subsurface ecosystem. Since the
publishing of this discovery in
2008, Ca. Desulforudis has been detected elsewhere in both
continental and marine subsurface. Single-cell genomic data suggests that
ancient viral infections transported archaea genes into Ca. Desulforudis cells,
which gave the bacterium the genetic machinery for its self-reliance.
Single-cell
genomic data has not only permitted us to investigate cell-to-cell variations
in the genomic materials of subsurface microbes, but also to recover the
genomic blueprints of microbes that cannot be cultivated. These overlooked
organisms are sometimes called ‘microbial dark matter’ because they evade
detection by conventional laboratory methods. As with astronomical dark matter,
microbial dark matter vastly exceeds the amount that is ‘visible’ to us. Some
99 per cent of the microorganisms do not grow under artificial laboratory
conditions. We must rely on single-cell genomics and metagenomics to hunt for
microbial dark matter in the deep subsurface.
Even after we
and several other research teams realised that bacteria and viruses have
colonised the harsh, deep subsurface, most scientists still considered it
unlikely that anything more complex than these unicellular organisms would be
able to survive down there. More complex, multicellular organisms generally
cope less well with low oxygen levels and high pressure, and they require more
food. All the same, in 2006 our group (led by Onstott and Gaetan Borgonie)
started to look for nematodes at great depths.
Nematodes
(commonly called roundworms, not to be confused with earthworms, which belong
to a group all of their own, the Annelida) are extremely common multicellular
organisms. Together with insects, they are the most dominant animals on the
planet. Nematodes are mostly very small. Although some can range up to several
metres in length, most are less than 1 mm long. Their origin extends back 1.1
billion years, to a time not long after the divergence of plants and animals in
evolution. Nematodes are considered to be among the oldest multicellular
organisms still known on the planet. They have conquered almost any niche on
the planet from soil to oceans; some have even evolved to parasitise plants and
animals, including humans.
What made
nematodes a logical choice to look for in the deep subsurface is their proven
track record for being able to survive in extreme environments. Many species
are able to alter their life cycle when confronted with life-threatening
conditions. They form a survival stage in which their metabolism is greatly
reduced. In this way, they are able to withstand anoxia, heat, drought,
freezing and toxic conditions for several decades, and then revive when wetted
or when conditions are adequate again.
Nematodes can
withstand huge pressures, too. When the Space Shuttle Columbia broke
up during re-entry in 2003, a biological experiment on board containing
nematodes made a free fall from an altitude as high as 42 km. Their canister
hit the ground with a force of roughly 2,500 g. (Transient centrifugation at up
to 10,000 gs, which would liquefy a human, is a common manipulation in standard
nematode laboratory procedures.) A few weeks later, the experiment was
recovered. The nematodes inside the canister had not only survived the ordeal,
they were reproducing. Furthermore, humans need 21 per cent oxygen in our
atmosphere to be able to breathe. Nematodes can make do indefinitely with only
0.5 per cent oxygen, and many species can survive extended periods with less or
no oxygen at all.
This effort
resulted in the discovery of a whole zoo of invertebrates in water that was
12,300 years old
Our search for
deep-Earth nematodes resulted in the 2011 discovery of
a new species of nematode, Halicephalobus mephisto. Its name literally
means ‘the devil worm’. The nematode was recovered from water that flowed out
of a fissure at a depth of about 1.3 km in the Beatrix gold mine. Carbon-dating
showed the water there to be around 3,000 years old. In the years that
followed, we found more nematodes living at an even more remarkable depth of
3.8 km.
After the
discovery of the devil-worm nematode, we performed a long filtration sampling
setup that lasted two years. During that time, we filtered 12,845,647 litres of
water at a depth of 1.4 km. (The search for deep life is painstaking work!)
This effort resulted in the discovery of a whole zoo of invertebrates in water
that was 12,300 years old. We recovered species of flatworms, nematodes,
rotifers, arthropods, annelids, fungi and protozoa, a whole community thriving
inside the filter.
Genetic analysis
revealed that none of these was a new species, but that they were all species
already known from the surface. Further investigation revealed that nearly all
the complex subsurface dwellers shared a common characteristic: they were known
to be cosmopolitan, and therefore well-suited to living in extreme
environments. At that time, we also made the first video footage of a biofilm –
a thin, self-contained living layer – attached to crevices deep inside the
rock. The biofilm is composed of bacteria and organic matrix, and it is home to
all these animals.
We also found
several non-animal species, such as fungi and protozoa, living in deep fissure
water that ranged in ages from 7,000 to 500,000 years old. Often their
abundance in the fissure water was low, just one specimen per 10,000 litres. In
contrast, in certain areas we found patches of bacterial biofilm containing
worms at population densities of more than 1,000,000 individuals per square
metre. Because the known subsurface animals are small, a cavity the size of
your thumb can hold an entire ecosystem containing several hundred small
invertebrates, fungi and protozoa.
The commonality
of species on the surface and subsurface posed a consistent research challenge.
At all times, we had to make extensive analysis to be sure that any specimen
found was not the result of contamination of the mines where we were executing
our research. We also measured the age of the water to be sure it was not
recent, using both chemical and bacteriological techniques. And we had to
maintain aseptic conditions at all times. These are similar to, though milder
than, the kinds of precautions that might soon be needed for analysing samples
from Mars for evidence of extraterrestrial life.
Except for Halicephalobus
mephisto, we never did find any completely new species of multicellular
organisms in the Beatrix mine. This seemed counterintuitive at first, as we
expected that a long process of adaptive selection in the deep subsurface would
lead to novel life forms. With the advantage of hindsight, though, it is not so
surprising.
If you consider
any patch of soil anywhere in the world, the nematodes (or any other small
invertebrate) living there undergo a daily and seasonal cycle of stress. On
bright days, sunshine can dry out the soil; when it rains, puddles might cut
off all oxygen; at night, the freezing of water or a bigger animal stepping on
that patch adds pressure and disturbs the soil. In summary, animals living in
the soil on the surface already experience stress every day. Many of the
organisms transported to the deep subsurface would have adapted to extreme
conditions long ago, so they would not need a long adaptive selection process
to be able to survive. That would account for the paucity of undiscovered deep
species.
It’s an enigma:
how did a salt-dependent surface worm get that deep without meeting deadly
fresh water?
Even after we
got past the surprise of what organisms we found living in the subsurface, we
were still caught off-guard by where we found them. During our survey
of the Beatrix mine, we discovered nematodes living inside salty stalactites at
a depth of about 1.4 km. Moreover, this species of nematode was adapted to
living in salty water and could not even survive in fresh water. On the
surface, this species had been found years before to live in brackish water
conditions. Although the Beatrix mine is situated in a dry salt pan, it is
still an enigma how a salt-dependent surface worm managed to get that deep
without encountering a deadly layer of fresh water in between.
The process of
transport to the deep subsurface is not yet understood, and is the subject of
much current research. Even in the absence of answers, the broader realisation
that complex surface life forms can also survive indefinitely in the deep
subsurface is good news for the search for life on planets and moons in our
solar system. A similar process of migration could have transported life forms
to the deep subsurface long before the surface conditions became inhospitable
on Mars, for instance.
And our journey
into the inner life of the Earth is just beginning. We are interested in
determining whether species from the deep subsurface truly are as isolated as
they seem, and if the migrations go in both directions. It is possible that
some subsurface organisms reappear on the surface via hot springs. Our analyses
of hot-spring waters in the Limpopo region as well as the southern and western
Cape regions of South Africa did not turn up any evidence of such resurfacing.
Nevertheless, this is a provocative issue that we are continuing to investigate
because it will tell us how frequently genetic materials are being exchanged
between the surface and the deep subsurface.
Finally, we
recognise that we have probably explored only a tiny fraction of the deep
biosphere, and might not yet have encountered its most significant inhabitants.
It stands to reason that, if cosmopolitan species from the surface can survive
in the deep subsurface, isolated from their surface brethren, then over a long
period of time some organisms might have adapted to even more extreme
conditions deeper in the subsurface. It could be that the real treasure trove
of new and weird life forms still awaits discovery far beneath our feet.
Reproduced from Aeon: :
https://aeon.co/essays/deep-beneath-the-earths-surface-life-is-weird-and-wonderful
